The most common pinout for the 2x5 motherboard connector consists of 2 rows of 4 (+1) pins, one row per port:
- pin 1: +5V
- pin 2: -data
- pin 3: +data
- pin 4: ground
- pin 5, if any: not connected / physical key for 4+5 connector, etc.
Some prehistoric motherboards (~5 years old) I have seen did not follow that layout.