Osaka is a vast city which has multiple train stations. You can take a train from Kansai Airport to Osaka Station, Namba Station, Tennoji Station or Shin-Osaka Station:
https://www.jrailpass.com/blog/kansa...rport_by_Train
Limousine bus services run frequently between Kansai Airport and a wide range of stops in Osaka including Osaka Station, Namba Station, Shin-Osaka Station, and Universal Studios. There are also night buses which run once an hour from the bus station between 12am and 5am. The journey takes one hour and the price is 1.550 yen.