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"If my wife and I have some points in our separate AA accounts, can I call AA and use both accounts to get two tickets for the same flight?"
No. But AA offers one-way awards. Does each of you have enough miles for a one-way?
If you need to combine miles from one account to another just to purchase a one-way award for one person, you would have to transfer miles from one account to another -- and that can be expensive.