Originally Posted by franxit

Keep in mind that any ticket purchased at a rock-bottom price (low cost and promo on Italo treno) will be not refundable.

Fares are very likely to disappear up 7 days before your intended travel date, the ones on Trenitalia will be definitely gone, while Italo usually has decent priced tickets up to 3 days before.

Tuscany is served by trenitalia, for regional travel (short distance) there's no reason to purchase tickets in advance, as Italian train prices are very low compared to the rest of Europe. If I were you I'd give italo business class a try on longer routes, as prices are very convenient and the price will be a fraction of what you'd pay on a flight.