Yes, the one you should take is State Road (SR) 528 (AKA The Beachline). You pick it up right by the airport. Take a look at this map of Orlando area toll roads:
https://www.oocea.com/Portals/0/Imag...essMap2009.pdf
It is faster timewise and there are fewer traffic hassles. Just have cash handy including some quarters. Stay to the
far right hand lane to pay in cash.