Sure.
Utilization is on a per-account and a total sum basis.
Let's say you've got two cards, and one has a $10k limit and one has a $25k limit.
You've got a $5k charge, and put it on the $10k card. That card is now at 50% utilization, and you will lose FICO points for that -- only for the time it's high, mind you, but that can cause adverse action if it's a big enough hit. I know from personal experience that, on a clean report, you can lose over 100 points for utilization. Ouch. Fortunately, it was a temporary situation when I didn't know better.
Most banks report to the credit bureaus as the statements cut, but not all do (HSBC reports at the end of the month).
If you know when that posts, then you can make sure that you've paid down your balance before the balance gets reported and thus avoid losing the FICO points.
For most people most of the time, you don't need to know this unless you're suddenly using credit or applying for credit.