Well, let's see: Chesley Burnett "Sully" Sullenberger III piloted Airbus A320 family aircraft, so he must have lost it, right?
Pilots are required to undergo simulator training and checks as well as cockpit checks with a check pilot on a regular basis, and required to recover aircraft from a variety of unusual attitudes, instrument losses, engine losses and other emergency conditions (simulators are often hair-raisingly realistic on these issues - pilots come out of the sim with a lot more sweat stains than they wen in with).
In a word, "no!"