Most of the car rental companies at RSW close by 2:00 AM and open back up at 4:00 AM or 4:30 AM. Enterprise actually has shorter hours than the others. They close at 12:30 AM and re-open at 5:00 AM. You might want to call one or two agencies and see if they would accommodate at 2:30 AM arrival. Usually there is at least one agency that stays open until the last flight arrives, but if your flight is running late or has been rescheduled past it's normal arrival time, you could find yourself stuck without anyone open.