I use a computer backpack on wheels and a large totebag (both private label from my company - totebag rotates according to what I like but I have one I've been using for months that we did for Disney Vacation Club - it has lots of pockets, is a zip top and is HUGE).
I pack WAY too much but I've had luggage lost too many times so I have to be a bit prepared:
Change of underwear
Change of clothes
Laptop
Extra Laptop battery
Empower kit
Portable DVD player
DVDs
Ipod
Noise reduction headphones
at least one book
3-4 magazines
2-3 snacks
breathmints & gum
Mouthwash
Olay cleansing cloths (the wet kind and dry kind)
Feminine products
Moisturizer
Eye Creme
Lip Balm
Foundation
Christian Dior travel cosmetic set (it has EVERYTHING!)
Cosmetic Brushes
Qtips
Medicines
Slipper socks
Clorox Wipes
Hand wipes
jewelry
mini Bible
Travel Photo frame
Travel Photo album
Travel cell phone in addition to regular phone and blackberry
Adaptor
And my purse w/ all the regular junk
Separate wallet that I keep foreign money in
Travel wallet with all travel info, foreign cards, etc.
Medical information (for my China trips I have a Chinese medical information card) with all allergy info
I'm sure I'm missing some stuff because my bags always seemed packed to the seams.